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Fig. 6. Blockade of Meis gene function inhibits RA-induced blastema proximalization. (A,B) Longitudinal sections through distal limb blastemas, 30 hours post-electroporation with rhodamine-coupled control MO (A) or fluorescein-anti-Meis1+Meis2 MOs (B). (C) Dorsal view of an axolotl amputated bilaterally at mid-carpal level, RA-treated, and electroporated with Meis1+Meis2 MOs (right) and control MO (left). (D) Skeletal preparations of forelimbs from a single animal regenerated after RA treatment and bilateral electroporation with control MO. (E) Skeletal preparations of forelimbs regenerated after RA treatment and electroporated with control MO (left) and Meis1+Meis2 MOs (right). Each pair is from a single animal. Numbers beneath forelimbs indicate duplication indexes. S, complete extra stylopod; s, partial extra stylopod; sh, extra shoulder girdle; Z, complete extra zeugopod; z, partial extra zeugopod.





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