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Fig. 1. Phenotypic analysis of GS cells. (A) Left, appearance of GS cells, 24
months after initiation of the cultures; middle, alkaline phosphatase activity
in the GS cells; right, appearance of mGS cells that closely resemble ES
cells. (B) Flow cytometric characterization of GS cells after 24 months in
culture. Black line, control immunoglobulin; red line, specific antibody. (C)
RT-PCR analysis. Specific primers were used to amplify cDNA from GS and mGS
cells. (D) Karyotype analysis of two separate cultures of GS cells. At least
50 cells were counted. (E) Cumulative growth curves for two separate cultures
of GS cells. Cells were maintained for 2 years in which the total number of
cells at each passage has been calculated. There is steady and consistent
exponential increase in total cell number over time. Scale bar: 100 µm in
A.
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