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Fig. 2. Analysis of telomere dynamics and telomerase activity. (A) Southern blot analysis of TRFs containing telomeric DNA sequences in five different ES (E14) cells and GS cells. Genomic DNAs from ES or GS cells were digested with HinfI, separated on a pulse-field gel and probed with the 5'-[32P](T2AG3)3 telomeric DNA oligonucleotides. (B) Loss of telomeres in GS cells. Based on the median values of the TRF analysis shown in (A), shortening rates of the telomeric DNA sequences in GS cells were calculated for two separate cultures. (C) Telomerase activity in ES and GS cells. ES and GS cells were subjected to a standard telomeric repeat amplification assay. Extracts were pretreated with (H) or without heat inactivation (80°C, 10 minutes) prior to the telomerase assay. The total amount of cell lysates per assay is indicated.





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