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Fig. 4. Spermatogenesis and the generation of offspring from GS cells.
EGFP-expressing GS cells from 24-month-old cultures were transplanted into the
seminiferous tubules of infertile W recipient mice. (A) A recipient testis
showing the EGFP fluorescence in GS cell-derived colonies. Green tubules
represent donor GS cell colonization. (B) Histological analysis (Hematoxylin
and Eosin staining) of the recipient testis showing normal-appearing
spermatogenesis. (C) Round spermatid released from a recipient testis,
exhibiting EGFP fluorescence (arrow). (D) Offspring resulting from the
microinjection of oocytes with round spermatids, exhibiting fluorescence.
Scale bar: 1 mm.