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Fig. 1. Generation of a telencephalic-specific deletion of Notch1. (A)
Coronal sections through the embryonic telencephalon of
Foxg1Cre/+; ROSA26-floxed-stop-lacZ reporter mice
stained with X-gal to visualize Cre activity. Cre-mediated recombination is
detected throughout the entire telencephalon by E9.5 and E10.5. (B)
Notch1 and Hes5 are present in the telencephalic VZ in
wild-type (WT) embryos, whereas Notch1 mRNA is not detected and
Hes5 is substantially diminished in the telencephalon of
Foxg1Cre; N1 cKOs at E10.5. Notably, residual Hes5
expression is detected only in the medial ganglionic eminence (asterisk), and
not in the striatal anlage, the lateral ganglionic eminence, which is more
laterally located. (C) At E12.5, Notch1 protein is not detected in
telencephalic lysates from Foxg1Cre; N1 cKOs (N1 cKO),
whereas levels of 
-tubulin are equivalent between N1 cKO and WT
forebrain lysates, as assessed by immunoblotting for the cleaved portion of
Notch1 and -tubulin. Western blot (WB): anti-Notch1 (N1) and
anti--tubulin (tub). (D) At E10.5, Notch2 is expressed at high
levels within the epithelium of the choroid plexus but is not detectable in
the VZ, whereas Notch3 is present at low levels throughout the VZ of
wild-type embryos. Scale bar: 150 µm.
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