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Fig. 7. Notch1 and Notch3 are not required for normal striatal development after cells exit the VZ. (A) A coronal section of an E12.5 Dlx5/6Cre-IRES-EGFP forebrain indicates that the Dlx5/6 enhancer directs the expression of EGFP throughout the ventral SVZ and mantle, but not in the VZ. (B) Dlx5/6Cre induces the recombination of floxed alleles throughout the striatum, including the SCS, patch and matrix compartments. Shown here is in a coromal section of a P1 striatum from a cross between the Dlx5/6Cre-IRES-EGFP transgenic and the Z/EG reporter mouse. Cells that have undergone Cre-mediated recombination permanently express EGFP (shown here in green). EGFP is expressed in all regions of the striatum at P1 in progeny from Dlx5/6Cre and Z/EG reporter mice. Antibodies to Darpp32 mark the SCS and patches (shown here in red). The EGFP-negative regions are fiber tracts passing through the striatum, with the anterior commissure (AC) visible in the lower left corner. (C) The SCS and patch compartments develop normally in Dlx5/6Cre; N1; N3 DKOs, as seen here with antibodies to Darpp32 at P1. (D) The matrix compartment is equivalent in both Dlx5/6Cre; N1; N3 DKOs and wild-type littermates at P1. Scale bar: 250 µm.

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