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Fig. 4. FGF signalling is required for stem zone specification. 3c and stage 7 CPM explant combination experiments (A). The ability of 7CPM to induce Sax1 (B,C) and Hoxb8 (D,E) is inhibited by exposure to SU5402 but not DMSO. Electroporation scheme at HH4 (F). Mis-expression of GFP only does not alter Sax1 expression (G,H), but that of DnFGFR1 inhibits Sax1 onset (red arrowheads, I-J'), while not reducing Sox2 (K-L').





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