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Fig. 5. FGF/MAPK signalling is required for stem zone maintenance. Electroporation scheme at HH10 (A). Mis-expression of GFP only does not reduce Sax1 expression (B-C'), while dnFGFR1 inhibits Sax1 (D-E'), but not Sox2 (F-G'). SU5402, but not DMSO, leads to loss Sprouty2 (H,I) and Sax1 (J,K), but not Sox2 expression (L,M). Similarly, PD184352, but not DMSO, reduces Sprouty2 (N,O) and Sax1 (P,Q), but does not attenuate Sox2 expression (R,S).





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