QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 7. NMDA receptor blockade specifically influences presynaptic sites without altering RGC axon arbor complexity. The effects of altering NMDAR transmission in the optic tectum on GFP-synaptobrevin cluster number and axon branching in tadpoles that received single tectal injections of APV or MK801 is shown as the percent change from their initial value at the time of treatment. (A) Both APV and MK801 significantly decreased GFP-synaptobrevin cluster number versus control 2 hours after treatment. The peak cumulative effects of APV on GFP-synaptobrevin cluster number occur 4 hours after treatment (0-4 hours), while the MK801 cumulative effects peak 6 hours after treatment (0-6 hours). (B) RGC axon arbor complexity, expressed as the increase in total branch number per axon terminal, is affected by the APV and MK801 treatments by 24 hours only. (C) A measure of synapse density is provided by comparing the change in GFP-synaptobrevin cluster number with the change in total arbor length from the initial observation. In controls, there is a one-to-one relationship in the increase in GFP-synaptobrevin cluster number to arbor length, while in APV and MK801-treated tadpoles GFP-synaptobrevin cluster density is significantly decreased to 50% or below. This difference is significant for all observation time points. n=14 axon arbors in control, n=10 in APV and n=12 in MK801. ^*P $≤$ 0.05; ^**P $≤$ 0.005; ^***P $≤$ 0.0005.

Return to article