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Fig. 9. BDNF prevents the acute effects of NMDAR blockade by maintaining GFP-synaptobrevin cluster addition and stabilization rates. (A,B) Detailed analyses of the number and distribution of GFP-synaptobrevin clusters per axon branch and of the lifetimes of individual presynaptic clusters for every observation period reveal the effects of BDNF on synapse dynamics following NMDAR blockade. APV significantly decreased the stability (A) of GFP-synaptobrevin clusters during the first 4 hours after treatment (0-2 and 2-4 hour observation periods). The proportion of GFP-synaptobrevin clusters that are stabilized in the presence of APV was significantly influenced by BDNF (APV+BDNF) during the first 2 hours of treatment. (B) A significant decrease in GFP-synaptobrevin cluster addition (0-2 and 2-4 hours) preceded an increase in cluster addition following APV treatment. BDNF rescued GFP-synaptobrevin cluster number by maintaining the rate of GFP-synaptobrevin cluster addition. GFP-synaptobrevin cluster dynamics is presented for APV-treated tadpoles, and was similar for axons in MK-801-treated tadpoles. The circled asterisks indicate significant differences between APV alone and APV+BDNF (P $≤$ 0.05). Double asterisks indicate significant differences between control and APV (P $≤$ 0.005). (A) APV+BDNF was significantly different from control at 0-2 and 2-4 hours only.

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