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Fig. 4. Prolonged Cut expression during mid-oogenesis caused defects in the mitotic
cycle/endocycle switch. Cut misexpression and Cut-String double misexpression
were achieved by the flip-out Gal4/UAS technique and are marked by
co-expression of GFP (green in A-G). All the clones were also marked by Cut
staining (not shown in the figure), except those in F. DAPI is shown in blue
(A-C,G) and white (A'). (A,A') Continuous expression of Cut during
mid-oogenesis caused the follicle cells to be more densely distributed and
their nuclei to be slightly smaller than those of the wild type. (B,B')
Cyclin A (shown in red in B and white in B') was found to be expressed
in some of the follicle cells misexpressing Cut in a stage 9 egg chamber,
whereas CycA was no longer present in wild-type cells. (C,C') ß-gal
expression (red in C and white in C') from a fzr enhancer trap
line fzrG0326 was downregulated in follicle-cell clones
with overexpression of Cut during mid-oogenesis. (D,D') ß-gal
expression (red in D and white in D') from stg-lacZ showed no
change in cut misexpressing clones during mid-oogenesis. (E,E')
PH3 (red in D and white in D') staining was found occasionally during
mid-oogenesis in follicle cells misexpressing both Cut and Stg.
(F,F',F'') Follicle-cell clones overexpressing Cut in stage 10B egg
chambers did not switch to the amplification stage. BrdU incorporation (red in
F and white in F') and Orc2 staining (blue in F and white in F'')
were not present in the clonal cells (outlined). (G,G') Overexpression
of Cut in a stage 10B egg chamber affected the uniform Cyclin E expression
pattern. No Cyclin E expression (red in G and white in G') was detected
in the clone misexpressing Cut (outlined).
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