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Fig. 7. Lens-specific regulation of Foxe3 promoter in transgenic mouse embryos. (A) The 6.2 kb promoter sequence upstream of the SmaI site (Brownell et al., 2000) was divided into four blocks, A-D, from the proximal side, and in combination ligated to a lacZ expression cassette, and primary transgenic embryos were produced. Panels in the middle show transgene expression in the head (lens and brain) under low magnification (left) and in the lens under high magnification (right). The numbers in the right panel indicate cases of transgene expression in transgene (PCR)-positive embryos. (B) Schematic presentation of Sip1/Smad8-responsive region, and major and minor lens elements in Foxe3 promoter.





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