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Fig. 1. Cerebellar neurospheres give rise to neurons that resemble distinct
cerebellar cell types in vitro. E14.5 (A-E) or adult (F-K) cerebellar
neurospheres expressing EGFP were plated on a cerebellar feeder layer and
allowed to differentiate. Cells derived from the neurospheres that express the
molecular marker indicated are double-labeled and appear yellow, whereas
neurosphere-derived cells not expressing the marker examined appear green,
while feeder-layer derived cells expressing the analyzed marker appear red. A
subpopulation of neurosphere-derived cells gave rise to GABA-ergic neurons
(A,F) and can be subdivided into the parvalbumin-expressing interneurons (B,G)
and the calbindin-positive Purkinje-like cells (C,H). Neurosphere-derived
cells also gave rise to neurons that resemble granule cells, as indicated by
their expression of glutamate (D,I) and TAG1 (E,J). The expression of
synaptophysin implies that the cells can generate synaptic vesicles in vitro
(K).
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