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Fig. 4. Cerebellar-derived neurospheres mainly give rise to astroglia when
transplanted to the forebrain. Constitutive EGFP-expressing adult
cerebellar-derived neurospheres were transplanted into the neonatal forebrain
(P4) and analyzed at P21. The grafted cells mainly remained in close proximity
to the injection site (A). GFAP-expressing cells were found in the corpus
callosum (B), in the cortex along the needle tract (C) and close to the
ventricle (D). TAG1-expressing cells were found close to the graft site (D).
Scale bars: 1 mm in A; 50 µm in B1,C1,D1,E1; 20 µm in B2,C2,D2,E2.
Although it appears that many of the GFP cells in B2-D2 are not expressing
GFAP, this is a result of the different localization of GFP and GFAP in these
cells. Reconstruction of optical stacks demonstrates that the GFP cells shown
in these figures are largely GFAP positive.
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