|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Embryonic and adult-derived cerebellar neurospheres give rise to neurons
with the characteristics of GABA-ergic interneurons and granule cells in vivo.
Constitutive EGFP-expressing E14.5- and adult-derived cerebellar neurospheres
were transplanted into the neonatal cerebellum (P4). A subpopulation of cells
was observed to integrate into the host cerebellum and express GABA (A-F),
indicating that they are interneurons. EGFP-positive calbindin-expressing
cells with the morphology of Purkinje cells were detected in the Purkinje cell
layer of E14.5-derived neurospheres, albeit at low frequency (G-I). In
adult-derived NSC transplantations, large fusiform cells within the Purkinje
cell layer that did not express calbindin were observed (J-L). Most commonly
observed were EGFP-expressing cells with the characteristics of granule cells.
These possessed a small cell body with three to five protruding dendrites and
were immunoreactive for glutamate (M-R). Current-voltage responses of immature
(S,U) and mature (T,V) GFP-positive cerebellar granule cells. Immature cells
exhibited long-duration, non-repetitive intermediate- and high-threshold
calcium spikes, whereas mature granule cells exhibit pronounced inward
rectification and fast, repetitive spiking. Synaptic input was not blocked.
Step protocol from initial step of –20pA, with 5pA increments. In T, the
+10pA step is not shown for clarity. Scale bars: 30 µm in C,F,R; 20 µm
in I,F,L. Horizontal bar, 50 milliseconds; vertical bar 10 mV in S,T;
horizontal bar, 200 mseconds; vertical bar, 10 mV in U,V.
|
