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Fig. 1. Immunolocalisation of BubR1 during syncytial divisions. In all images, DNA is stained blue and BubR1 is stained red. (A-E) BubR1 immunolocalisation during syncytial nuclear division cycles 3-9 and (F-J) cycles 10-13. (A,F) BubR1 shows no specific localisation during interphase but (B,G) starts to accumulate at the kinetochores in prophase, (C,H) reaching a maximum in prometaphase, (D,I) decreases at metaphase and (E,J) is absent at anaphase. (K,K''') High-magnification view of a syncytial nucleus in prophase showing co-localisation of BubR1 and CID (shown in green). (K) Chromosome morphology at prophase showing initiation of condensation. (K') BubR1 co-localises with (K'') CID at kinetochores of prophase chromosomes. (K''') Merged image showing colocalisation of BubR1 and CID. (L,L''') High-magnification view of a syncytial nucleus at metaphase showing co-localisation of BubR1 over spindle microtubules (green). (L) Chromosome morphology at metaphase showing alignment along the metaphase plate. (L') At metaphase, BubR1 is sometimes detected along spindle microtubules (see also inset in D). (L'') Spindle microtubules were detected with anti--tubulin antibodies. (L''') Merged image showing co-localisation of BubR1 along spindle microtubules. Scale bar: 10 µm.

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