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Fig. 1. Cross-regulation of PIN expression and function in embryo development. (A-C) Novel embryo phenotypes in pin1,3,4,7 multiple mutants (C) compared with wild-type (A) and pin1,7 mutant (B) embryos. (D-G) Immunostaining showing that PIN4 is ectopically expressed in the suspensor of the pin7 preglobular embryo (G) in a pattern similar to that of PIN7 expression in wild type (F). No PIN4 expression in wild type at this stage (D) and expression restricted to root meristem precursors at the later stages (E). For the embryo stages the numbers indicate the developmental stage according to the actual number of pro-embryo cells of the corresponding wild-type stage. G, globular; H, heart; l, late; T, Torpedo; Tr, triangular; y, young.





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