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Fig. 2. Cross-regulation of PIN gene expression in root. (A) Symbolic depiction of PIN gene expression and polar localization in preglobular, early heart embryos and in seedling root. Arrows indicate presumed directions of auxin flow based on subcellular PIN polarity. (B) Comparison of root and meristem length between pin1 and pin2 single mutants and pin1,2 double mutants. Standard deviations are depicted. (C) Seedling phenotypes of wild type, pin2 single mutant and pin1,2 double mutants. (D-L) Immunostaining showing the cross-regulation of PIN expression. PIN1 is upregulated in the epidermis of pin2 root (E) compared with wild type (D). Detail showing polar PIN1 localization in epidermis of pin2 root (F). PIN2 is ectopically expressed in the stele of pin1 root (H) compared with wild type (G). Detail showing polar PIN2 localization in pin1 stele (I). PIN4 is upregulated in the stele of pin1 root (K,L) compared with wild type (J). Arrows indicate corresponding expression domains; arrowheads polarity of PIN localization.





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