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Fig. 3. Manipulation of auxin homeostasis leads to ectopic PIN gene expression.
(A-H) Inhibition of auxin transport by NPA (50 µmol/l for 24 hours) leads
to upregulation of PIN1::PIN1:GFP in epidermis and cortex (B);
PIN2::PIN2:HA (D), PIN4::PIN4:GFP (F) and PIN4::GUS
(H) in the stele compared with untreated controls (A,C,E,G). (I-L) Treatment
for 24 hours with biologically active auxins such as IAA (50 µmol/l, I),
2,4-D (0.1 µmol/l, J) and NAA (10 µmol/l, K) or for 5 days with auxin
precursor sirtinol (20 µmol/l, L) leads to an upregulation of
PIN4::GUS expression compared with control (G). (M,N) Upregulation of
PIN2::GUS expression in root following treatment with 50 µmol/l
NAA for 24 hours (N) compared with untreated control (M). (O,P) Treatment for
5 days with auxin precursor sirtinol (20 µmol/l) leads to upregulation of
PIN1::PIN1:GFP in epidermis and cortex cells (P) compared with
control (O).
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