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Fig. 5. Time- and concentration-dependence of auxin-regulated PIN gene expression. (A-D) Upregulation of PIN1::GUS (A), PIN3::GUS (B), PIN4::GUS (C) and PIN7::GUS (D) expression after different times and different concentrations of 2,4-D incubation. Inset (D) shows untreated PIN7::GUS roots after prolonged GUS staining. (E) Downregulation of PIN7:GUS fusion protein abundance in PIN7::PIN7:GUS seedlings.





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