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Fig. 6. Quantitative evaluation of auxin-regulated PIN gene expression. (A,B) Quantitative RT-PCRs showing time-dependence (A) and concentration-dependence (B) of the effect of auxin (1 µmol/l 2,4-D in A) on PIN expression. Insets show higher magnifications of early time points (A) and low concentrations (B). The legend in B also applies to A. (C,D) Auxin (1 µmol/l 2,4-D) does not induce PIN expression in slr-1 mutants (C) or after induction of axr3 expression in HS::axr3-1 lines (D), as shown by quantitative RT-PCR. Cycloheximide alone induces PIN expression (C). (E,F) Northern blot (E) and quantitative GUS assays (F) show the time-dependence of auxin (10 µmol/l NAA) effect on PIN2 expression. (G) An expression profiling experiment shows auxin-dependent upregulation of PIN genes, PID and selected primary auxin response genes in differentiated parts of the root grown on NPA. The auxin-dependent upregulation of PIN gene expression is abolished in the slr1 mutant. Induction of PIN gene expression is depicted relative to the non-induced controls.





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