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Fig. 1. Generation of mice harboring floxed Brg1 alleles and characterisation of the Cre activity of K14-Cre mice during embryogenesis. (A) Targeting strategy of the mouse Brg1 locus. Partial structure of the Brg1 locus [Brg1 (+) allele]. Exons are indicated by black boxes. The Brg1 L3 allele containing the three loxP sites (lox) and the hygromycin resistance marker (Hygro) is presented. The expected genomic maps after excision of the floxed marker (Brg1 L2 allele) and excision of both the marker and exons 2 and 3 (Brg1 L^- allele) are shown. The arrows indicate the PCR primer location (P1-P3). The location of the Southern blot 5'-probe (Sumi-Ichinose et al., 1997) is shown. The size of DNA segments obtained after BamHI restriction digest and detection with the 5'-probe are indicated. B, BamHI; N, NheI; X, XbaI; E, EcoRV. (B) Southern blot analysis of genomic DNA isolated from Brg1^+/+, Brg1^L3/+ and Brg1^L2/+ ES cells, digested by BamHI and hybridized with the 5' probe. (C) Whole-mount X-Gal stained E9.5 (a,b) and E10.0 (c) control ROSA^fl/+ (a) and K14-Cre^tg/0/ROSA^fl/+ (b,c) embryos. fl, forelimb; hl, hindlimb. (D) X-Gal-stained dorsal skin sections from E12.5 (a,b), E14.5 (c,d) and E18.5 (e,f) control ROSA^fl/+ (a,c,e) and K14-Cre^tg/0/ROSA^fl/+ (b,d,f) fetuses. Sections were counterstained with safranin. E, epidermis; D, dermis. Scale bar in a: 24 µm.

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