QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Selective ablation of BRG1 in the surface ectoderm and epidermal keratinocytes of K14-Cre^tg/0/Brg1^L2/L2 fetuses. (A) Immunohistochemical (IHC) detection (green fluorescence) of Brg1 (a-h) on E11 forelimb (a,b,e,f) and hindlimb (c,d,g,h) bud tranverse sections from control (a,c,e,g) and Brg1^ep-/-(c) (b,f,d,h) fetuses. Superimposition of Brg1 (green fluorescence) and DAPI staining of nuclei (blue fluorescence; e-h). Scale bar in a: 24 µm. (B) IHC detection of Brg1 on transverse sections through the dorsal ectoderm of E12.5 Brg1^L2/L2 (control; a,c) and Brg1^ep-/-(c) (b,d) fetuses. Superimposition of Brg1 (green fluorescence) and DAPI staining of nuclei (blue fluorescence; c,d). Scale bar in a: 24 µm. (C) IHC detection of Brg1 on dorsal skin sections from (a,b) control Brg1^L2/L2 and (c,d) Brg1^ep-/-(c) E18.5 fetuses. Red staining corresponds to BRG1 antibody and blue staining to DAPI. E, epidermis; D, dermis. The dotted line indicates the dermal-epidermal junction. Scale bar in a: 10 µm. (D) PCR detection of the Brg1 L2 and L^- alleles in the epidermis and dermis of E18.5 fetuses. DNA was extracted from tail epidermis (E; lanes 1,3,5,7,9) or dermis (D; lanes 2,4,6,8,10) of fetuses with the indicated genotypes, isolated from females that were Tam treated from either E9.5-E13.5 (lanes 3,4) or E12.5-E16.5 (lanes 5-8), or oil (vehicle) treated from E12.5-E16.5 (lanes 9,10).

Return to article