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Fig. 4. Characterisation of skin defects in Brg1^ep-/-(c) fetuses. (A) Histological analysis of Toluidine Blue-stained semithin sections of dorsal skin biopsies from E18.5 Brg1^L2/L2 (a,c) and Brg1^ep-/-(c) (b,d) fetuses. E, epidermis; D, dermis. Epidermal cell layers are indicated: C, cornified; G, granular; S, spinous; B, basal. Scale bars: in a, 60 µm for a,b; in c, 18 µm for c,d. (B) Scanning electron microscopy of the dorsal skin surface from E18.5 Brg1^L2/L2 (a) and Brg1^ep-/-(c) (b) fetuses. Scale bar: 10 µm. (C) Transmission electron microscopy of dorsal skin from E18.5 Brg1^L2/L2 (a,c,e,g) and Brg1^ep-/-(c) (b,d,f,h) fetuses. (a,b) Stratum granulosum (SG). D, desmosomes; KF, keratin filaments; KG, keratohyalin granules. Arrows indicate lamellar granules (LG). Insets show LGs. (c,d,e,f) Junction between stratum granulosum (SG) and stratum corneum (SC). Large black arrows in c and e point towards the LG(s) in the SG-SC interface, and arrowheads in e point towards aligned extracellular lipid discs at the junction between the granular and the cornified layer. The white arrow in c,d indicates desmosomes (D); small black arrows in d,f indicate vesicles at the interface of SG and SC. (g,h) Consecutive C1, C2, C3 and C4 cornified cell layers. Black arrows in h point towards vesicles. Black and white brackets in g and h indicate lipid lamellae (LL). CD, corneodesmosomes. Scale bar in a: 0.2 µm for a,b,c,d; 0.1 µm for e,f; and 0.25 µm for g,h.

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