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Fig. 5. Staining for neutral lipids and analysis of genes involved in epidermal barrier formation in skin from Brg1^ep-/-(c) fetuses. (A) Nile Red staining for neutral lipids in the skin of E18.5 control (a) and Brg1^ep-/-(c) fetuses (b). Arrowheads in b indicate an absence of neutral lipids. Scale bar in a: 16 µm. C, cornified layer; SB, suprabasal layer; B, basal layer. (B) Quantitative RT-PCR analysis of skin RNA from E18.5 Brg1^L2/L2 (lanes 1 and 3) and Brg1^ep-/-(c) (lanes 2 and 4) fetuses. (C) Real-time RT-PCR analysis of corneodesmosin on skin RNA from E18.5 Brg1^L2/L2 and Brg1^ep-/-(c) fetuses. ^**P<0.001.

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