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Fig. 7. Characterisation of fetuses after temporally controlled Brg1 ablation in the forming epidermis. (A) Gross morphology of (a,c) control Brg1^L2/L2, (b) Brg1^ep-/-(ia) and (d) Brg1^ep-/-(ib) E18.5 fetuses after daily Tam administration to pregnant females from E9.5-E13.5 (a,b) and E12.5-E16.5 (c,d). (B) Histological analysis of Toluidine Blue-stained semithin sections of dorsal skin biopsies from (a) control Brg1^L2/L2 and (b) Brg1^ep-/-(ib) E18.5 fetuses. C, cornified layer; G, granular layer; S, suprabasal layer; B, basal layer; D, dermis. Scale bar in a: 18 µm. (C) Transmission electron microscopy of stratum corneum (SC) (C1,C2 and C3 consecutive cornified cell layers) from dorsal skin of (a) Brg1^L2/L2 and (b) Brg1^ep-/-(ib) E18.5 fetuses. White arrows and white arrowheads point towards corneodesmosomes (CD) and lipid lamellae (LL), respectively. Scale bar in a: 0.25 µm. (D) X-Gal diffusion assay on (a) control Brg1^L2/L2 and (b) Brg1^ep-/-(ib) mutant E18.5 fetuses. (E) TEWL measured on E18.5 control Brg1^L2/L2 and Brg1^ep-/-(ib) fetuses. ^***P<0.001.

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