|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. AprA is secreted by starving Dictyostelium cells, but is not part
of the CF complex. Conditioned starvation medium from wild-type cells was
fractionated by sieving gel filtration chromatography. Western blots of the
fractions were stained with affinity-purified rabbit antibodies against CF50,
countin and AprA. On the SDS-polyacrylamide gels, the upper band stained with
anti-countin antibodies is at 40 kDa, the CF50 band is at 50 kDa and the heavy
AprA band is at 60 kDa. The affinity-purified anti-AprA antibodies stained a
band at 
56 kDa in fractions 28-30. Besides this band and the heavy band
at 60 kDa, the anti-AprA antibodies did not stain any other band. See
Fig. 3 for validation that the
anti-AprA antibodies are staining the product of the aprA gene.
|
