|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. AprA slows the proliferation of cells. (A) Cells were diluted to
2x105 cells/ml in HL5 and the cell density was measured
daily. The graph shows means±s.e.m. from three independent experiments.
WT indicates wild type. The insert shows the data from the first two days
plotted using a log scale for the density. The saturation densities (in units
of 107 cells/ml) were 3.24±0.06 for
aprA-, 1.94±0.07 for wild type and 0.69±0.10
for aprA-/actin15::aprA. The differences between the
wild-type saturation density and either the aprA- or the
aprA-/actin15::aprA saturation density were significant
(P<0.01; one-way ANOVA; Dunnett's test). (B) For each cell type,
three 100 mm petri dishes with SM/5 agar were spread with a lawn of bacteria
mixed with 1000 Dictyostelium cells. At the indicated times, the
bacteria and cells were washed off one of the plates, and the
Dictyostelium cells were counted. The graph shows means±s.e.m.
from three independent experiments. At 72 hours, the difference between wild
type and aprA-/actin15::aprA was significant
(P<0.05) and the difference between wild type and
aprA- was significant (P<0.01; one-way ANOVA,
Dunnett's test). The absence of error bars indicates the error was smaller
than the plot symbol.
|
