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Fig. 4. Indirect induction of Chordin by Xnr1 and ß-catenin.
Induction of Chordin by ß-catenin (A) and by Xnr1 (B) is
inhibited by cycloheximide. RNA (100 pg) encoding Xnr1 or constitutively
active ß-catenin was injected into Xenopus laevis embryos at the
one-cell stage and animal caps were dissected at mid blastula stage 8 (before
the mid blastula transition). The animal pole regions were allowed to develop
for 3 hours in the presence or absence of cycloheximide and RNA was analysed
by quantitative RT-PCR for expression of Chordin. Induction of
Chordin by both Xnr1 and ß-catenin is inhibited by cycloheximide
and is therefore indirect.
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