Supplemental Figure 1
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Fig. S1. Loss of Fgf8 and Fgfr signaling leads
to a general increase in cell death in the developing eye. (A-D) Acridine
Orange (AO) staining reveals cell death in the optic vesicle of live embryos at
the 20-somite stage (incubation: 10- to 20-somite stage). Low level cell death
in wild type (A), increased cell death in ace mutants (B) and strong cell death in embryos treated with Fgfr
inhibitor (C). Rescue of cell death in ace after Fgf8-soaked bead implantation; the outline of the bead is
indicated (D). (E-G) Analysis of apoptosis by TUNEL at the 20-somite stage
shows low-level cell death in a wild-type optic vesicle (E), an intermediate
number of cells in an ace optic
vesicle (F) and a high number in an optic vesicle from an
Fgfr-inhibitor-treated embryo (G); there is no bias of cell death to the
proximal optic vesicle (future nasal retina). (A-D) Maximum-intensity
projections of 10 confocal sections through the head region, orientation as
shown in A. (E-G) Outlines represent margin of the optic vesicle. NT boundary
in E,F was plotted on the basis of efna5a and epha4b expression at
the same developmental stage. A, anterior; P, posterior; Pr, proximal; Di,
distal; os, optic stalk; ov, optic vesicle; die, diencephalon; N, nasal; T,
temporal.
