|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. UNC-43 CaMKII activation in the proximal gonad. (A) The rat sequence used
to generate CaMKII-pT is highly conserved in C. elegans UNC-43,
including the phosphorylated threonine (*, T284 in UNC-43 and T286
in rat CaMKII). (B-D) CaMKII-pT stains the oocytes of wild-type gonads. In
mid-focal plane images, staining co-localizes with F-actin at the oocyte
cortex. (E-G) CaMKII-pT staining is not observed in
unc-43(n1186)-null gonads. (H-J) At the sheath and oocyte interface
of wild-type gonads, CaMKII-pT staining is observed in the same focal plane as
sheath cell F-actin. (K) In contrast to CaMKII-pT, MAPK-YT staining is
uniformly distributed throughout the cytoplasm, and sometimes in the nucleus.
(L) CaMKII-pT stains sheath cells surrounding the sperm in fem-3(q20)
gonads. The genetic requirements of UNC-43 T284 phosphorylation are shown in
M-U. (M) No CaMKII-pT staining is observed in unmated fog-3(q443)
females, indicating that sperm are required for UNC-43 T284 phosphorylation.
(N,O) CaMKII-pT stains unmated fog-2(q71) female gonads microinjected
with 100 nM MSP (N), but not buffer-injected control gonads (O). (P) CaMKII-pT
staining is not observed in unmated vab-1 RNAi ceh-18(mg57)
females, which undergo constitutive oocyte maturation independent of sperm
presence. CaMKII-pT staining is not observed in vab-1(dx31) (Q) and
nmr-1(ak4) gonads (S), but it is observed in vab-1(e2)
kinase dead (R), itr-1(sa73) (T) and itr-1(sy290gf) (U)
gonads. All images in B-M were processed with deconvolution software and
include DAPI to visualize DNA. All gonads are oriented as shown in
Fig. 1A. Scale bars: 10
µm.
|
