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Files in this Data Supplement:
Movie 1. Second cleavage divisions in which both two-cell blastomeres are labelled with the beads at their vegetal poles. In the two-cell stage blastomere undertaking an E-division, membrane labelled by the bead does not become displaced. As a result, after the equatorial division the bead is associated with the four-cell stage blastomere that is positioned distal to the polar body, the e2 cell.
Movie 2. Second cleavage divisions in which both two-cell stage blastomeres are labelled with the beads at their vegetal poles. In the meridionally dividing two-cell stage blastomere, membrane labelled by the bead does not become displaced and thus it remains at the vegetal pole. However, in the other, two-cell stage blastomere that undertakes an E-division, the vegetal membrane labelled with the bead becomes displaced towards the future plane of the division. Ultimately, the four-cell stage blastomere is positioned more proximal to the polar body; the e1 cell is labelled with the bead.
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