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Fig. 6. The role of Rho-family GTPases in mesoderm spreading. (A) A section through
an embryo that lacks the function of Rac2. The mesoderm flattens down
onto the ectoderm as in wild type, but does not adhere to the ectoderm
properly. (B) The effect of reducing the maternal dose of RhoA. The
interaction of the mesoderm with the ectoderm is not affected, but the shape
of the cells in the mesoderm is abnormal. (C,D) Embryos derived from mothers
with reduced dose of (C) Rac1 and Rac2, or (D) Rac1,
Rac2 and Mtl. In these mutants, contact between the mesoderm and
the ectoderm fails to be established properly. (E) Embryos that lack the
materal and zygotic function of the Rac effector Pak show no effect
upon the initation of mesodermal spreading. (F) A section of a homozygous
pebble mutant embryo. Contact of the mesoderm with the ectoderm does
not occur, and MAPK activation is not observed within the mesoderm at this
stage of development. Embryos shown in A-E were stained with Twist and the
embryo in Fwas stained with anti-dp-ERK.
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