DEV01596 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Sequence alignments suggest that Tbx5 and Tbx20 morpholinos cannot crossreact. (A) Alignments of Tbx5 morpholinos (complementary sequence) with Tbx5 and Tbx20 mRNA sequence. (B) Alignments of Tbx20 morpholinos (complementary sequence) with Tbx5 and Tbx20 mRNA sequence.

• Supplemental Figure 2 -

  Fig. S2. Tbx5 is expressed in the common cardinal and anterior hepatic veins. (A,B) Whole-mount in situ hybridization of Xenopus embryos at stage 36 (A) and 42 (B). Parasaggital section show expression in the myocardium (m), endocardium (e) and pericardium (p). (C,D) Oblique sections through a stage 36 embryo showing expression in the common cardinal (cc) and anterior hepatic veins (h).

• Supplemental Figure 3 -

  Fig. S3. Tbx5 and Tbx20 morpholinos block the formation of a functional heart as assayed by benzidine staining of erythrocytes in stage 42 tadpoles. (A) Uninjected control, (B) TBX5MO injected at optimal dose, (C) TBX20MO injected at optimal dose, (D) TBX5MO and TBX20MO injected together at suboptimal doses. There is blood staining in the heart region (h) of control embryos but not in morphant embryos.