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Fig. 4. XCL100 and U0126 inhibit decondensation of sperm chromatin after pronuclear fusion. Hoechst 33342 dye DNA staining of control embryos, embryos microinjected with 2.5 µM recombinant XCL100 and embryos incubated with 100 µM U0126. The times indicated in the Control panels apply to all three conditions. Arrows indicate sperm chromatin within zygote nucleus. Inset at 120 minutes shows the control embryo undergoing cytokinesis, while the XCL100- and U0126-treated embryos have not undergone NEB. Scale bar: 50 µm.





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