|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Notum suppresses Wg signaling mainly through Dally. (A-D) Hnt staining
(red) in a wild-type disc (A), a homozygous dally disc (C) and discs
carrying clones mutant for notum (B) or dally-notum (D).
Wg-dependent Hnt expression marks sense organ precursor (SOP) cells, which
will give rise to bristles on the future wing margin. In the wild-type disc,
SOP cells are in two rows abutting Wg-expressing cells. Each row contains
20-22 cells. In the clone mutant for notum, Hnt is ectopically
expressed such that the rows are doubled, while in the dally disc,
the number of SOP cells in each row is reduced to 9-11. In the clone mutant
for dally-notum, the number of SOP cells is also reduced. (E-H)
Anterior wing margins of wild-type adult (E), notum escaper (F),
dally escaper (G) and a wing carrying large clones of
dally-notum double mutant (marked by yellow colour) (H). The dorsal
view of the wild-type wing margin reveals two rows of sensory bristles:
densely packed stout mechanosensory bristles and interspersed chemosensory
bristles. Three chemosensory bristles are indicated by red arrows to show the
spacing. The wing of the notum escaper has many ectopic bristles of
both types, while that of dally has a reduced number of chemosensory
bristles, as visualized by the increased spacing (red arrows). The
dally-notum clone does not have ectopic bristles but has increased
spacing between chemosensory bristles (red arrows). (I,J) Hnt staining in
discs carrying large clones mutant for either dlp (I) or
dlp-notum (J). Occasionally, few ectopic SOP cells can be seen in
dlp clones (white arrows), while the dlp-notum clone has
many ectopic SOP cells. (K,L) Anterior wing margins of
dlpA187/A203 escaper (K)
and a wing carrying a large clone of dlp-notum double mutant (L). The
dlp wing has a few ectopic stout mechanosensory bristles (black
arrows), while many ectopic bristles of both types were produced in the
dlp-notum clone.
|
