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Fig. 4. Sequential dissociation of the epithelial DM and formation of its
derivatives as revealed by morphology and Pax7 mRNA expression.
Transverse sections in-situ hybridized with PAX7 (blue) and immunolabeled with
desmin (brown). (A) The epithelial DM at E3 expresses PAX7, but the underlying
myotome is devoid of PAX7+ cells. (B) At E3.5 the central DM partially
dissociates into a compact cell aggregate (ID) that retains PAX7 expression.
Few PAX7+ cells become apparent in the myotome but no dermis is yet apparent.
(C) By E4 three distinct domains are clearly visible, the desmin+ myotome
containing PAX7+ cells, the compact ID, which expresses PAX7, and the
beginning of a PAX7-negative dermis composed of scattered mesenchymal cells.
See also a similar expression pattern of PAX3 between the myotome and dermis
in Fig. 3A. (D) At E5 the ID
domain has almost disappeared and most PAX7+ cells are in the myotome
intermingled among desmin+ fibers and also located medial to them. A well
developed dermis is present. D, dermis; DM, dermomyotome; ID, intermediate
domain; M, myotome; NT, neural tube. Scale bar: 40 µm in A; 55 µm in B;
60 µm in C; 120 µm in D.
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