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Fig. 5. Mesp1/Mesp2 double-null cells fail to contribute to epithelial somites or
to the dermomyotome. (A-D) Tail regions from X-gal-stained whole-mount
specimens of control (A,C) and double-null (B,D) chimeric embryos. (A,B)
Lateral view. (C,D) Dorsal view. The blue double-heterozygous cells are
randomly distributed in the control embryo, whereas the Mesp1/Mesp2
double-null cells are excluded from the lateral region of the somites
(arrowheads in D). (E,F) Parasagittal sections of tails from chimeric embryos.
(E) The labeled cells are randomly located in the control chimera. (F) The two
types of cells are randomly mixed in the PSM, whereas the dermomyotome-like
epithelium consisted exclusively of wild-type cells and the sclerotome-like
compartment contained mostly Mesp1/Mesp2 double-null cells. Note that normal
epithelial somites are not formed in this chimera. (G,H) Transverse sections
show elimination of Mesp1/Mesp2 double-null cells from the dermomyotome. (I,J)
The dermomyotome-like epithelium in the Mesp1/Mesp2 double-null chimeric
embryo gives rise to dermomyotome, myotome (arrowhead in J) and the dorsal
part of the sclerotome. Red arches indicate the inner surface of dermomyotome.
(K,L) AlexaFluor 488-labeled phalloidin staining shows normal
epithelialization of somites in the control chimera (K) and restriction of
epithelialization in the dermomyotome-like compartment in the Mesp1/Mesp2
double-null chimera (L). dm, dermomyotome; dml, dermomyotome-like epithelium;
dsc, dorsal part of the sclerotome; my, myotome; nt, neural tube; sc,
sclerotome; scl, sclerotome-like compartment; tg, tail gut.
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