|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Expression of Sax1 in the nucleus of the mlf. (A) Schematic
lateral view of the embryonic mesencephalon and pretectum, showing the
expression domains of Sax1 in blue, and indicating major axon tracts
and the location of their cell bodies. The fully developed expression pattern
of Sax1 is depicted as seen in embryos from HH21. (B) Lateral view of
the MFB of an HH27 chick embryo, with retrograde labelling of the mlf (DiI,
red) from the rostral hindbrain and the pc (DiO, green) from the dorsal
pretectum (see red and green asterisks, respectively, in A), showing the
partial overlap of the corresponding nuclei for these axon tracts originating
from the ventral MFB. (C) Lateral view of chick head at HH17, with the
Sax1 expression domains in rhombencephalon (arrowhead), mesencephalon
(arrow) and pretectum in blue. (D) Lateral view of the ventral mesencephalon
of an HH23 embryo. Sax1 mRNA signals are in blue, and the red
staining shows the mlf, retrograde-labelled with fluorescein-labelled dextran
from the rostral hindbrain (see red asterisk in A). The nucleus of the mlf
(arrows) overlaps with the Sax1 expression domain. (E-H)
Double-labelling for Sax1 mRNA in blue and neurofilament protein in
brown. Lateral views of the ventral mesencephalon of an HH17 (E) and an HH21
(F) embryo show that the Sax1 domain overlaps with the cell bodies
for the mlf (arrow in E). At HH21, Sax1 staining is weakly visible in
the dorsal pretectum. (G) A horizontal section through the ventral
mesencephalon of an HH22 embryo (boxed area magnified in H) shows
Sax1-expressing neurons projecting into the mlf (arrows). The MFB is
marked by a line in D,F,G. INC, interstitial nucleus of Cajal; mes,
mesencephalon; mlf, medial longitudinal fascicle; nIII, oculomotor nucleus;
pc, posterior commissure; pt, pretectum; r1, rhombomere 1.
|
