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Fig. 6. A neurula stage graft of notochord and floorplate rescues Barhl2 gain-of-function phenotype. (A) Notochord and floorplate explants from stage 12-14 ß-galactosidase (100 pg)-injected donor embryos were grafted in the presumptive neural plate midline of wild-type or mouse Barhl2 (25 pg)-injected embryos. (B-D) The embryos were analyzed at stage 40. ß-Galactosidase activity is revealed in blue. (B) Control embryo (top) and control embryo grafted with wild-type explants (bottom). (C) Mouse Barhl2-injected embryos (n=40). (D) Mouse Barhl2-injected embryos grafted with explants from ß-galactosidase-injected wild-type donors (n=41).





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