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Fig. 3. Notch affects the levels of Armadillo and of ArmadilloS10. (A-D)
Effects of TNotch on the stability of ArmadilloS10 and wild-type
Armadillo in third instar wing discs (anterior down and posterior up);
expression is under the control of dppGAL4 at 22°C. (A)
Expression of ArmadilloS10. (B) Expression of TNotch with
ArmadilloS10; notice the reduction in the overall amount of
ArmadilloS10. (C) Expression of wild-type Armadillo. Armadillo
(red) is very unstable and is only stabilised in the presence of Wingless
(green; see also Fig. S3 in supplementary material). (D) Expression of
wild-type Armadillo with TNotch. The ectopic Armadillo has been eliminated
except for a small amount in the neighbourhood of the Wingless-expressing
cells (see inset) and this is not associated with a loss of Wingless
expression. (E,F) Western blots showing the concentration of endogenous
Armadillo and ArmadilloS10 in the presence or absence of various
forms of Notch, expression is under the control of dppGal4 (F) or
C5Gal4 (E). In the presence of Armadillos10 an elevation of
endogenous Armadillo levels (E) or an increase in the hypophosphorylated form
(lower band of doublet, F) is observed compared to wild type. Expression of
TNotch under the control of dppGal4 results in a marked decrease of
both Armadillo and Armadillos10 (due to insufficient separation
Armadillo doublet is visualised as one band in E), whereas expression of
TNotch under the control of C5Gal4 results in a reduction of
hypophosphorylated form of endogenous Armadillo and of Armadillos10
(F). The effects of full length Notch (Notch) are less marked, under the
control of dppGal4 Notch expression results in a decrease of
endogenous Armadillo (E); and with C5Gal4 a reduction in
Armadillos10 is apparent. (F). Expression of NICD results in small
increases in the amounts of Armadillos10 (E,F).
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