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Fig. 7. Armadillo associates with Notch in Drosophila embryos. Notch
protein was immunoprecipitated from wild-type embryos and the presence of
associated proteins was assessed by western blot. The majority of Notch
protein present in embryo lysate and immunoprecipitated was the full-length
protein (>250 kDa) which, in Drosophila is the predominant form of
Notch at the cell surface (Kidd and
Lieber, 2002). Detected in association with immunoprecipitated
Notch were both Armadillo (middle panel) and Dishevelled (upper panel)
protein. The lane labelled `lysate' represents a fifth of the total protein
added to the immunoprecipitation reaction. Immunoprecipitated Notch reflects
about 5-10% of the total Notch and associated with this is 0.5-3% of the total
Armadillo and less than 0.5% of the total Dishevelled. Given that the pool of
Armadillo associated with Notch is not associated with E-cadherin it is not
surprising that this Notch-associated fraction of Armadillo is only a small
proportion of the total cellular Armadillo.
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