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Fig. 2. The onset of anaphase is regulated by both Cdk1-CycB activity and the
Pim-Thr-Sse complex in a dose-dependent manner. The durations of cell-cycle
phases are measured by analyzing timelapse recordings using two-photon
microscopy in embryos with different levels of CycB and different components
of Pim-Thr-Sse complex between cycles 6 and 11. The duration of
prophase-metaphase and anaphase-telophase do not change between cycle 6 and 11
(Ji et al., 2004), thus data
are averages of the duration from 10-20 time-lapse recordings of each genotype
between cycle 6 and 11. Prophase-metaphase duration is 220 seconds in
wild-type embryos (51 measurements from 20 embryos, s.d.=30 seconds); 270
seconds in four cycB embryos (51 measurements from 18 embryos,
s.d.=40 seconds); 350 seconds in thr1/four cycB embryos
(24 measurements from 11 embryos, s.d.=60 seconds); 240 seconds in
pim1/four cycB embryos (33 measurements from 10 embryos,
s.d.=30 seconds) and 230 seconds in sse13m/four cycB
embryos (52 measurements from 11 embryos, s.d.=30 seconds). When compared with
four cycB embryos, the differences caused by lower thr, pim
or Sse levels are significant (P<0.0002). There is no
significant difference in the duration of anaphase-telophase in the five
genotypes (duration varies between 160-180 seconds).
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