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Fig. 1. Functional analysis of the chicken DC5(8x) enhancer in Drosophila.
(A) Wild-type and mutant sequences of the DC5 enhancer used in this study. SOX
and PAX6 binding sites are indicated. Altered nucleotides are shown in red.
(B) Scheme of the construct used to test the functionality of the DC5 enhancer
in Drosophila. The octamerized DC5 enhancer was cloned upstream of a
minimal promoter (MP) and the EGFP reporter gene. (C-I) Activity pattern of
the DC5(8x) enhancer in the adult Drosophila head. Enhancer activity
was detected in the compound eye when the wild-type sequence was used (C). The
mutant M4 and M7 enhancers failed to drive EGFP expression (D,E), although the
transgenesis marker used (3xP3-DsRed1) was equally expressed in the three
cases (F,G,H), indicating that the chromosomal insertion point of the
different constructs did not affect enhancer functionality. (I) Enhancer
activity is also detected in the adult antenna (red arrow), the maxillary
palps (blue arrow) and the labial palps (yellow arrow). (J-L) During larval
development, the DC5(8x) enhancer is active in the eye imaginal disc (J) and
in Bolwig's organ (K,L).
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