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Fig. 6. DC5(8x) activity in the Drosophila compound eye is abolished by
depletion of either D-Pax2 or SoxN. (A) The DC5(8x) enhancer
is inactive in Drosophila spapol mutant. This mutant is
characterized by the absence of D-Pax2 expression in cone cells and
primary pigment cells. Nevertheless, cone cells do still form in
spapol mutant, as shown by means of the cone-cell-specific
enhancer SME (B). (C-F) SoxN depletion was studied using the clonal
analysis technique. Mutant clones in the adult compound eye were generated
using the strategy depicted in panel E. SoxN clones
(red arrows in C,D,F) were identified by the lack of red pigment
(w) under white illumination (C). The use of UV
light facilitated the identification of the mutant tissue (D). The activity of
the DC5(8x) enhancer is abolished in the SoxN
clones (F).