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Fig. 6. DC5(8x) activity in the Drosophila compound eye is abolished by depletion of either D-Pax2 or SoxN. (A) The DC5(8x) enhancer is inactive in Drosophila spapol mutant. This mutant is characterized by the absence of D-Pax2 expression in cone cells and primary pigment cells. Nevertheless, cone cells do still form in spapol mutant, as shown by means of the cone-cell-specific enhancer SME (B). (C-F) SoxN depletion was studied using the clonal analysis technique. Mutant clones in the adult compound eye were generated using the strategy depicted in panel E. SoxN clones (red arrows in C,D,F) were identified by the lack of red pigment (w) under white illumination (C). The use of UV light facilitated the identification of the mutant tissue (D). The activity of the DC5(8x) enhancer is abolished in the SoxN clones (F).





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