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Fig. 3. Chick organizer graft experiments. (A-C) Chick organizer graft experiments
showing changes in inducing ability with increasing age of the donor. (A,C)
Dorsal views of early quail donor embryos. (B) Dorsal view of host chick
embryo, which simultaneously receives a graft of a quail stage 4 node on its
left and a quail stage 6 node on its right. Both grafts are placed in the
extra-embryonic area opaca (brown), just outside the embryonic area pellucida
(yellow). (D) Results of this experiment after in situ hybridization for the
hindbrain marker Krox20 (purple), which is expressed in rhombomeres 3
and 5 (upwards arrow), and after staining with an anti-quail antibody
(reddish-brown). The young (stage 4) graft has induced a complete axis
including the head (expressing Krox20), while the older graft on the
right has generated a short axis, mostly derived from the graft itself
(reddish-brown indicating quail cells), which lack rostral structures,
including the hindbrain (Krox20-expressing region). (A-C) Modified,
with permission, from Stern (Stern,
2004). (D) Reproduced, with permission, from Storey et al.
(Storey et al., 1992).
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