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Fig. 6. ZLI formation in D1/2 explants varies with the levels of Hh signaling applied. Stage 13-14 D1/2 explants were cultured in presence of varying concentrations of Hh-Ag1.3 and the relative length of the ZLI was assessed after 48 hours in culture by Shh (black) and Lfng (red) expression. Exposure to 250 (B), 500 (C) and 1000 (D) nM Hh-Ag1.3 resulted in increasingly longer domains of Shh expression in D1/2 explants than in untreated controls (A). (E) Quantitation of the dose effect of Hh-Ag1.3 addition on the relative length of the Shh domain in the ZLI. Error bars represent s.e.m.





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