DEV01798 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Expression of Dcc in late-born neurons of E12.5, E13.5 and E14.5 spinal cords. (A) Weak Dcc staining is detected in the dorsal aspect of the neural tube at E12.5 (arrow). (B,C) No Dcc staining is detected in the superficial dorsal horn at E13.5 (arrow in B) or E14.5 (arrow in C). Asterisks indicate the central canal. DH, dorsal horn; df, dorsal fasciculus; lf, lateral fasciculus. Scale bars: 100 mm

• Supplemental Figure 2 -

  Fig. S2. Comparison of the dorsal spinal cord between wild-type and Unc5h3^–/– mutant mice at P10. (A,B) Calbindin 28K staining reveals the abnormal disruption of laminar I-II cells in the medial superficial dorsal horn (arrow in B). (C-F) Peripherin 57K (C,D) and parvalbumin (E,F) staining shows the abnormal projection of proprioceptive afferents in the medial superficial dorsal horn (arrows in D,F). Scale bars: 100 mm in A-D; 200 mm in E,F.

• Supplemental Figure 3 -

  Fig. S3. Abnormal entry site of primary afferents in Unc5h3^–/– mutant mice at E15.5. DiI labeling shows abnormal entry site of proprioceptive afferents in the dorsal horn of Unc5h3^–/– (B,B¢), which is more clearly shown by reducing exposure time (B¢). In wild-type mice, proprioceptive afferents are seen in the most superficial region of the dorsal horn (A¢), but they are abnormally present within the superficial dorsal horn in Unc5h3^–/– mutant (arrow in B¢). The projection of proprioceptive afferents to the ventral horn is slightly reduced in Unc5h3^–/– (arrowhead in B¢), compared with wild-type mice (arrowhead in A¢). Broken white lines outline the ventral horn of the spinal cord.  Scale bars: 100 mm.