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Fig. 4. Expression of early-born neuron-specific markers in wild-type and
Dcc–/– mutants. (A,B) Math1
immunocytochemical staining of E10.5 wild-type (A) and
Dcc–/– mutant (B) dorsal neural tubes shows an
absence of Math1+ cells in more ventral aspect of the
neural tube of Dcc–/– mutant (arrowhead in B)
when compared with wild-type control (arrowhead in A). (C,D) Foxd3
(dI2 marker) expression in E10.5 wild-type (C) and
Dcc–/– mutant (D) neural tubes detected by in
situ hybridization. Foxd3+ cells are absent in more
ventral aspect of the neural tube, as indicated by arrowhead in D. (E,F)
Isl1 staining of E11.5 wild-type (E) and
Dcc–/– mutant (F) neural tubes. More
Isl1+ cells are present in the dorsal part of the neural
tube (arrow in F). (G,H) Double staining of Lmx1b (green; dI5 marker)
and Pax2 (red; dI4, dI6 marker) in E10.5 wild-type (G) and
Dcc–/– mutant (H) neural tubes. Arrowhead in H
indicates dorsally migrated Lmx1b+ cells. (I,J)
Phox2a staining of E10.5 wild-type (I) and
Dcc–/– mutant (J) neural tubes. Arrows and
arrowheads in I,J indicate normal and abnormal positions of
Phox2a+ neurons, respectively. Scale bars: 100 µm.
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