|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Abnormal projection of proprioceptive afferents in
Dcc–/– mutants at E18.5. (A,B)
Immunocytochemical staining of Lmx1b shows the distribution of
Lmx1b+ neurons in the superficial dorsal horn of the
wild-type mice (A), and the region lacking Lmx1b+ neurons
(arrow in B) in the dorsal horn of Dcc–/–
mutants at E18.5. (C,D) 2H3 staining shows the absence of 2H3+
primary afferents to the ventral horn (double arrowhead in D) and its abnormal
location in the superficial dorsal horn (arrow in D) in
Dcc–/– mutants (D), compared with wild-type
mice (C). (E,F) TrkA immunostaining shows the absence of TrkA+
primary afferent in the medial superficial dorsal horn in
Dcc–/– mutants (arrow in F). In wild-type
mice, TrkA+ fibers are evenly distributed in the superficial dorsal
horn (E). (G,H) DiI labeling shows a dramatic reduction of proprioceptive
projection to the ventral horn (large arrow in H) and abnormal projection to
the midline region (small arrow in H) of
Dcc–/– mutant, compared with wild-type mice
(G). Broken white lines outline the ventral horn of the spinal cord. Scale
bars: 100 µm.
|
